Background:
Because the anaerobic bacteria are very fastidious to grow and difficult to isolate, recommended specimens for anaerobic culture are inoculated onto nonselective and selective media, which are difficult to comply in most clinical laboratories
in
Korea. We evaluated the effect on the isolation of anaerobic bacteria, with the addition of nonselective media.
Methods:
Nonselective brucella blood agar (BBA), selective phenylethanol blood agar (PEBA) and Bacteroides bile esculin agar (BBEA) were inoculated with 1, 100 clinical pus specimens, and the isolation rates of anaerobes were compared according to the
primary culture media.
Results:
number of anaerobe strain isolated were 93 when PEBA only was used, but it increased by 47% with the addition of BBA, while it increased by 58% with the addition of both BBA and BBEA.
Conclusions:
As a minimal set of media, we recommend the inoculation of a supplemented thioglycollate broth, and one each of selective and nonselective media to increase the isolation rate of anaerobes.
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